Air pollution is one of the five leading risk factors for mortality worldwide. India has been dealing with seriousenvironmental problems as its economy develops. The number of automobiles on the road has increased the risk of air pollution.Therefore, to reduce pollution levels, Government of India decided to leapfrog Bharat Stage V (BSV) (equivalent to Euro V)emission standards and directly implement the stricter and more comprehensive Bharat Stage VI (BSVI) (equivalent to Euro VI)emission regimen. One of the features of BSVI emission standard is E20 fuel i.e., 20% ethanol blending in fuel. This study aimedto correlate the levels of heavy metals and chemicals in urine as well as pro-inflammatory biomarkers in serum with air qualitycomparing BSVI and BSIV regions.